bmi 1 mouse monoclonal antibody  (Santa Cruz Biotechnology)

Journal: Cancer Research Communications

Article Title: Tumor Microenvironment Landscapes Supporting EGFR-mutant NSCLC Are Modulated at the Single-cell Interaction Level by Unesbulin Treatment

doi: 10.1158/2767-9764.CRC-23-0161

Figure Lengend Snippet: BMI-1 inhibition affects cell cycle progression and tumor growth. A, Western blot analyses of the human H1975 cell line treated for 24, 48, and 72 hours with DMSO Vehicle as control, and Unesbulin or PTC-028. Protein lysates were immunoblotted with an anti-BMI-1 or an anti-Tubulin antibody. Loading was assessed with an anti-β-actin antibody. Expected size is shown in kDa. The slower migrating hyperphosphorylated BMI-1 band is indicated by the arrowhead. B, Cell cycle analysis of the H1975 cell lines after treatment for 24 hours with DMSO (black), Unesbulin (light blue), or PTC-028 (dark blue). The bar charts represent the distribution of cells in G 0 , G 1 , and G 2 –M phases. P values are indicated. Error bars represent SD. Percentages of cells in each cell cycle phase are indicated. C, H1975 xenografts tumor volumes at treatment termination (Vehicle n = 15, black; Unesbulin n = 17, light blue; PTC208 n = 8, dark blue) normalized to the tumor volume measured at the beginning of treatment. The difference in tumor size at day 21 was statistically significant ( P values are indicated). D, The graph shows percentage of change in transgenic mice tumor volume measured by MRI at the indicated timepoints, between Unesbulin ( n = 8, light blue) and Vehicle-treated ( n = 6, black) groups. Error bars represent SD. P value is indicated. E, Confocal microscopy fluorescence nuclear imaging of DNA and H2AK119ub in tumor tissues collected at the end of treatment. Top panels show a representative image of DMSO-treated tumor; bottom panels show a tumor treated with Unesbulin. Left panels show DNA staining by Hoechst 33342 (blue acquisition channel). Right panels show H2AK119ub staining by immunofluorescence (scale bar, 10 µm).

Article Snippet: IHC were performed on paraffin sections with an anti-BMI-1 mouse monoclonal antibody (Millipore, #05637; 1/100 dilution) on mouse tissues.

Techniques: Inhibition, Western Blot, Cell Cycle Assay, Transgenic Assay, Confocal Microscopy, Fluorescence, Imaging, Staining, Immunofluorescence

Journal: BMC Gastroenterology

Article Title: Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma

doi: 10.1186/1471-230X-12-146

Figure Lengend Snippet: High Bmi-1 expression in various histologic types by immunohistochemical studies. A . Bmi-1 positive cells predominantly in the basal layer of normal esophageal squamous epithelium; B . Distribution of Bmi-1 positive cells mostly at the base of glands in columnar cell metaplasia; C . Bmi-1 positive cells mostly at the base of glands in intestinal metaplasia; D . Distribution of Bmi-1 positive cells evenly in the glands of low-grade dysplasia glands.

Article Snippet: After endogenous peroxidase activity was quenched and nonspecific binding was blocked, ready-to-use mouse monoclonal antibody anti-Bmi-1 (Millipore, MA) was incubated at room temperature for 30 minutes.

Techniques: Expressing, Immunohistochemical staining

Journal: BMC Gastroenterology

Article Title: Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma

doi: 10.1186/1471-230X-12-146

Figure Lengend Snippet: Immunohistochemical score of Bmi-1 in esophageal adenocarcinoma (EAC). A . No Bmi-1 expression in EAC glands (score 0); B . Bmi-1 weakly positive cells distributed evenly in EAC glands (score 1+); C . Bmi-1 moderately positive cells distributed evenly in EAC glands (score 2+); D . Bmi-1 strongly postive cells are distributed in mostly at the base of EAC glands (score 3+).

Article Snippet: After endogenous peroxidase activity was quenched and nonspecific binding was blocked, ready-to-use mouse monoclonal antibody anti-Bmi-1 (Millipore, MA) was incubated at room temperature for 30 minutes.

Techniques: Immunohistochemical staining, Expressing

Journal: BMC Gastroenterology

Article Title: Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma

doi: 10.1186/1471-230X-12-146

Figure Lengend Snippet: Immunohistochemical score of Bmi-1 in esophageal squamous cell carcinoma (ESCC). A . No Bmi-1 expression in ESCC (score 0); B . Bmi-1 weakly positive cells distributed evenly in ESCC (score 1+); C . Bmi-1 moderately positive cells distributed evenly in ESCC (score 2+); D . Bmi-1 strongly positive cells in ESCC (score 3+).

Article Snippet: After endogenous peroxidase activity was quenched and nonspecific binding was blocked, ready-to-use mouse monoclonal antibody anti-Bmi-1 (Millipore, MA) was incubated at room temperature for 30 minutes.

Techniques: Immunohistochemical staining, Expressing

Journal: BMC Gastroenterology

Article Title: Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma

doi: 10.1186/1471-230X-12-146

Figure Lengend Snippet: Kaplan-Meier analysis of overall survival associated with high Bmi-1 expression and in esophageal adenocarcinoma (A) and squamous cell carcinoma (B). A . Bmi-1 expression in EAC showed a better but not significant overall survival ( p =0.13). B . Bmi-1 expression in ESCC showed no change. Solid line: non-high expression; Dotted line: high expression.

Article Snippet: After endogenous peroxidase activity was quenched and nonspecific binding was blocked, ready-to-use mouse monoclonal antibody anti-Bmi-1 (Millipore, MA) was incubated at room temperature for 30 minutes.

Techniques: Expressing

Journal: BMC Gastroenterology

Article Title: Clinicopathologic characteristics of high expression of Bmi-1 in esophageal adenocarcinoma and squamous cell carcinoma

doi: 10.1186/1471-230X-12-146

Figure Lengend Snippet: Frequency histogram showing amplification of the Bmi-1 locus at chromosome 10p12 in 116 esophageal adenocarcinoma samples. This locus was amplified in 4/116 (3%) cases of this patient cohort (darker green).

Article Snippet: After endogenous peroxidase activity was quenched and nonspecific binding was blocked, ready-to-use mouse monoclonal antibody anti-Bmi-1 (Millipore, MA) was incubated at room temperature for 30 minutes.

Techniques: Amplification